Microfluidic Design Optimization Using Tilted Baffles for Efficient mRNA-LNP Formulation

📅 June 4, 2025

RNA therapeutics are transforming modern medicine—but clinical success depends on the precise, scalable formulation of lipid nanoparticles (LNPs). This study introduces a novel microfluidic platform for fabricating GFP mRNA-loaded LNPs using PDMS-based chips with customized channel geometries.

Through computational fluid dynamics (CFD) simulations and experimental validation, a 70° tilted rectangular baffle with a length of 150 μm was identified as the optimal configuration for mixing efficiency and particle uniformity. The resulting LNPs achieved maximum transfection efficiency at an N/P ratio of 5.6, demonstrating excellent performance in gene delivery applications.

This work lays the groundwork for scalable, high-throughput LNP manufacturing, enabling rapid development of RNA-based vaccines and therapeutics. A next-generation plastic microfluidic chip platform is currently under development to industrialize the process, further supporting clinical translation of nanomedicines through faster screening and robust manufacturing.

🔗 Read the full article in ACS Biomaterials Science & Engineering: https://lnkd.in/gFweYdtp

🔬 Keywords  

#RNATherapeutics #LipidNanoparticles #Microfluidics #mRNADelivery #Nanomedicine #CFDSimulation #BiomedicalEngineering #mRNAVaccine #TransfectionEfficiency #GFPmRNA #PDMSChip #OncologyTherapeutics #VaccineDevelopment #ScalableBiomanufacturing #LabOnAChip #RNAFormulation #NextGenMedicine #BiotechInnovation #ClinicalTranslation